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Image Search Results
Journal: Viruses
Article Title: New Detection Methods for Cryphonectria Hypovirus 1 (CHV1) through SYBR Green-Based Real-Time PCR and Loop-Mediated Isothermal Amplification (LAMP).
doi: 10.3390/v16081203
Figure Lengend Snippet: Figure 3. (a) Melting curve and (b) melting peak analysis of the qPCR products, a single melting peak at 82.5 ◦C, (c) amplification curve for sensitivity determination with 10-fold dilution series (DS) of the cDNA starting from 10 ng to 100 fg cDNA/µL (lines 1 to 6), (d) PCR products of DS for ORF B with 12F and 12R primers, (e) PCR products of DS for ORF A with hvep1 and hvep2 primers, (f) LAMP reaction products for DS, (g) qPCR amplifications of DS with the primer pairs CHV-F/CHV-R (119 bp). M: 100 bp DNA ladder (Solis BioDyne, Tartu, Estonia). Line 7: CHV1-free isolates (EU1), and Line 8: RNase-free ddH2O.
Article Snippet: M: 100 bp
Techniques: Amplification